| 赵雪松,任新,段小月,罗州颖,朱蓉.全氟辛烷磺酸盐暴露对斑马鱼胚胎发育毒性与氧化应激的影响[J].唐山学院学报,2016,29(6):12-16 |
| 全氟辛烷磺酸盐暴露对斑马鱼胚胎发育毒性与氧化应激的影响 |
| On the Effect of PFOS on Developmental Toxicity and Oxidative Stress in Zebrafish Embryos |
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| DOI：10.16160/j.cnki.tsxyxb.2016.06.004 |
| 中文关键词: 全氟辛烷磺酸盐(PFOS) 斑马鱼胚胎 发育 氧化应激 |
| 英文关键词: perfluorooctanesul phonic acid potassium salt(PFOS) zebrafish embryos development oxidative stress |
| 基金项目:国家自然科学基金项目（21507039）；吉林省教育厅“十三五”科学技术研究项目（吉教科合字[2016]第227号） |
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| 中文摘要: |
| 探讨了全氟辛烷磺酸盐（PFOS）对斑马鱼胚胎的发育毒性与氧化应激的影响。将斑马鱼胚胎暴露于系列浓度（0.1 mg/L，0.5 mg/L，1 mg/L，5 mg/L，10 mg/L）的PFOS中，分别在胚胎受精后数小时（24 hpf，48 hpf，72 hpf，96 hpf，120 hpf）内统计急性发育毒性指标，包括存活率、畸形率、心率以及孵化率。同时在暴露结束的120 hpf，检测相关的抗氧化酶活性变化，主要包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（Gpx）以及脂质过氧化物（MDA）。结果显示，PFOS能够导致胚胎/幼鱼存活率降低，畸形率增加，心率加快以及孵化延迟；同时，胚胎SOD活性以及MDA水平增加，CAT与Gpx活性降低，表明PFOS能够诱发斑马鱼胚胎急性发育中毒，影响其抗氧化系统，导致胚胎产生氧化应激。 |
| 英文摘要: |
| This paper is aimed at studying the effect of PFOS on developmental toxicity and oxidative stress in zebrafish embryos. In the experiments done by the authors, the zebrafish embryos were exposed to PFOS(0.1 mg/L,0.5 mg/L,1 mg/L,5 mg/L,10 mg/L), and acute developmental toxicity indexes at different hours after fertilization(24 hpf,48 hpf,72 hpf,96 hpf,120 hpf)were collected, including survial rate, malformation rate, heart rate and hatching rate. At the end of 120 hpf exposure, the changes in antioxidant enzyme activity were examined, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx) and lipid peroxidation Oxide (MDA). The results indicate that PFOS tends to decrease the survival rate of embryos and larvae, increase the rate of malformation, speed up the heart rate and delay the hatching. They also show that the activities of SOD and MDA in the embryo increase and those of CAT and Gpx decrease, indicating that PFOS can induce the acute developmental toxicity of zebrafish embryos, affect the antioxidant system and lead to embryonic oxidative stress. |
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